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Cell line nameB16-F10
Accession numberICLC ATL99010
Brief descriptionSpecies: mouse, C57BL/6; Tumor: melanoma
DescriptionSpecies: mouse, C57BL/6; Tumor: melanoma
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperCancer Res 1975;35:218-224 - PMID: 1109790
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesThe cells produce melanin
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with NP, PepB
Tumorigenicitytumorigenic and metastatic in syngeneic animals
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyNature 1973;242:148-149

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