Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | JM2 |
Accession number | ICLC ATL99009 |
Brief description | Species: rat, Fisher 344; Tissue: liver; Tumor: hepatoma |
Description | Species: rat, Fisher 344; Tissue: liver; Tumor: hepatoma |
Depositor | Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy |
Reference paper | In Vitro 1983, 19:191-202 - DOI: 10.1007/BF02618059 - PMID: 6131863 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2 x 104 cells/cm2; 37C, 5% CO2 |
Properties | drug metabolism assays, hepatocyte studies, toxicology; low basal and prooxidant-induced lipid peroxidation. Reduced alcohol dehydrogenase (ADH) and glutathione S-transferase (GST) activities; increased aldehyde dehydrogenase (ALDH) and aldehyde reductase (ALDR) activities when 4-hydroxynonenal (4-HNE) is used as substrate |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as rat with NP, PepB |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Further bibliography | Cancer Res 1984, 44:5219-26 - PMID: 6488182
Cancer Res 1988, 48:7009-7012 - PMID: 3263898 Carcinogenesis 1994, 15:1359-64 - DOI: 10.1093/carcin/15.7.1359 - PMID: 8033312 Free Radic Biol Med 1999, 26:333-340 - DOI: 10.1016/s0891-5849(98)00206-8 - PMID: 9895224 |