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Cell line nameJM2
Accession numberICLC ATL99009
Brief descriptionSpecies: rat, Fisher 344; Tissue: liver; Tumor: hepatoma
DescriptionSpecies: rat, Fisher 344; Tissue: liver; Tumor: hepatoma
DepositorProf. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Reference paperIn Vitro 1983, 19:191-202 - DOI: 10.1007/BF02618059 - PMID: 6131863
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2 x 104 cells/cm2; 37C, 5% CO2
Propertiesdrug metabolism assays, hepatocyte studies, toxicology; low basal and prooxidant-induced lipid peroxidation. Reduced alcohol dehydrogenase (ADH) and glutathione S-transferase (GST) activities; increased aldehyde dehydrogenase (ALDH) and aldehyde reductase (ALDR) activities when 4-hydroxynonenal (4-HNE) is used as substrate
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with NP, PepB
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCancer Res 1984, 44:5219-26 - PMID: 6488182
Cancer Res 1988, 48:7009-7012 - PMID: 3263898
Carcinogenesis 1994, 15:1359-64 - DOI: 10.1093/carcin/15.7.1359 - PMID: 8033312
Free Radic Biol Med 1999, 26:333-340 - DOI: 10.1016/s0891-5849(98)00206-8 - PMID: 9895224

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