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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | B104 |
| Accession number | ICLC ATL99008 |
| Brief description | Species: rat, BDIX; Tissue: brain; Tumor: neuroblastoma |
| Description | Species: rat, BDIX adult; Tissue: brain; Tumor: neuroblastoma |
| Depositor | Dr F. Tosetti, Molecular Biology Dept IST at ABC, Genoa, Italy |
| Reference paper | Nature 1974;249:224-227 - DOI: 10.1038/249224a0 - PMID: 4151463 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast and neuroblast, small cells |
| Culture conditions | DMEM (4,5 g/L) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; split confluent cultures 1:10-1:20 using trypsin/EDTA (2-3 min.); seed at 1-3 x 104 cells/cm2; 5% CO |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Brain Res 1981;254:563-575 - DOI: 10.1016/0165-3806(81)90024-9
- PMID: 6458347
J Neurochem 1999;72:2286-2293 - DOI: 10.1046/j.1471-4159.1999.0722286.x - PMID: 10349837 Proc Natl Acad Sci USA 1979;76:514-517 - DOI: 10.1073/pnas.76.1.514 - PMID: 284369 |