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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | Neuro-2A |
| Accession number | ICLC ATL99007 |
| Brief description | Species: mouse, albino; Tumor: neuroblastoma |
| Description | Species: mouse, albino; Tumor: neuroblastoma |
| Depositor | Dr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy |
| Reference paper | J. Cell Biol. 1969, 43:69A; Proc: Natl Acad. Sci. USA 1970, 65:129-136 |
| Morphology and growth | monoclonal continuous culture, grown as monolayer, morphology neuronal-like and round, mostly loosely attached cells, some in suspension |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split subconfluent cultures 1:3-1:6; seed at 2-4 x 104 cells/cm2; 5% CO2, 37°C |
| Properties | the cells are reported to produce large quantities of microtubular protein, which plays a role in a contractile system which is responsible for axoplasmic flow in nerve cells |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |