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Cell line nameSR-4987
Accession numberICLC ATL99005
Brief descriptionSpecies: mouse, BDF/1; Tissue: bone marrow, stromal cells; Transformed by: murine leukemia virus
DescriptionSpecies: mouse, BDF/1 female 5 months old; Tissue: bone marrow, stromal cells; Transformed by: murine leukemia virus
DepositorDr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy
Reference paperCytotechnology 1992;8:93-102 - DOI: 10.1007/BF02525491 - PMID: 1382506
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesthe cells are reported to produce macrophage-colony stimulating factor (M-CSF) and are highly sensitive to fibroblast growth factor (FGF)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, Pep B
Passage number197
Tumorigenicitytumorigenic in syngeneic animals
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyExp Hematol 1997;25:536-541 - PMID: 9197333
Methods Mol Cell Biol 1995;5:230-236

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