Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | WEHI-3B/CPX |
Accession number | ICLC ATL99004 |
Brief description | Species: mouse, BALB/c; Tissue: monocyte; Tumor: leukemia, myelomonocytic |
Description | Species: mouse, BALB/c; Tissue: monocyte; Tumor: leukemia, myelomonocytic |
Depositor | Dr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy |
Reference paper | Cancer J 1993;6:291-297 |
Morphology and growth | continuous culture, grown in suspension, morphology small, round, single cells |
Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; maintain cultures between 1-5 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
Properties | the cells produce IL-3; resistant to cyprofloxacin |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as mouse with MD, PepB |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |