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Interlab Cell Line Collection (ICLC)
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Cell line nameFAO
Accession numberICLC ATL99001
Brief descriptionSpecies: rat; Tissue: liver; Tumor: hepatoma, H-35 Reuber
DescriptionSpecies: rat; Tissue: liver; Tumor: hepatoma, H-35 Reuber; Derived from: H4-II-E-C3
DepositorProf. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Reference paperBiochimie 1974;56:1603-1611 - DOI: 10.1016/s0300-9084(75)80286-0 - PMID: 4157008
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesuseful for studying regulation of monooxygenase activities and analyzing their role in the activation of xenobiotics (carcinogens, drugs and pesticides)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyEur J Biochem 1984;145:455-462 - DOI: 10.1111/j.1432-1033.1984.tb08577.x - PMID: 6334605

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