ICLC logo Cell bank
IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line nameHepa 1-clc7
Accession numberICLC ATL98016
Brief descriptionSpecies: mouse, C57L; Tissue: liver; Tumor: hepatoma, BW7756
DescriptionSpecies: mouse, C57L; Tissue: liver; Tumor: hepatoma, BW7756; Derived from: Hepa-1cl, cloned from the Hepa-1 cell line
DepositorProf. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Reference paperDev Biol 1973;35:83-96 - DOI: 10.1016/0012-1606(73)90008-0 - PMID: 4362668
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesthe cells possess the aryl hydrocarbon (Ah) receptor and are highly inducible for cytochrome P4501A1; drug metabolism assays
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, Pep B
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyBiochimie 1991;73:61-66 - DOI: 10.1016/0300-9084(91)90075-c - PMID: 1851644
Proc Natl Acad Sci USA 1979;76:373-376 - DOI: 10.1073/pnas.76.1.373 - PMID: 106390
Science 1980;209:497-499 - DOI: 10.1126/science.6248960 - PMID: 6248960

For any info contact the Interlab Cell Line Collection
HTML by Paolo Romano