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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | B104-1-1 |
| Accession number | ICLC ATL98015 |
| Brief description | Species: mouse, Swiss albino; Tissue: embryo; Transfected with: DNA from rat neuroblastoma B104; Original line: NIH/3T3 |
| Description | Species: mouse, Swiss albino; Tissue: embryo; Transfected with EcoR1 digested DNA from the rat neuroblastoma B104; Original line: NIH/3T3 |
| Depositor | Dr U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy |
| Reference paper | Nature 1984;312:513-516 - DOI: 10.1038/312513a0 - PMID: 6095109 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | the cells contain the transforming gene neu which induces synthesis of a tumor antigen of relative molecular mass 185,000 (p185), strongly associated with the presence of neuro/glioblastoma oncogenes. The neu oncogene bears homology to erb-B and the tumor antigen p185 is serologically related to the epidermal growth factor receptor |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |