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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | PC-12 |
| Accession number | ICLC ATL98004 |
| Brief description | Species: rat; Tissue: adrenal; Tumor: pheochromocytoma |
| Description | Species: rat; Tissue: adrenal; Tumor: pheochromocytoma |
| Depositor | Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy |
| Reference paper | Proc Natl Acad Sci USA 1976;73:2424-2426 |
| Morphology and growth | continuous culture, grown in suspension, morphology clusters of small cells |
| Culture conditions | RPMI + 5% FBS + 10% HS + 2mM L-Glutamine; split 1:3-1:6; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2 |
| Properties | the cells are described to synthesize catecholamines (dopamine, norepinephrine); respond reversibly to NGF by induction of the neuronal phenotype. |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Science 1985;229:393-395 - DOI: 10.1126/science.3839317
- PMID: 3839317
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| Comments | to obtain single cells, pass the suspension through a 22G syringe; use collagen-coated flasks for attachment |