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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | P388D1 clone 3124 |
| Accession number | ICLC ATL97013 |
| Brief description | Species: mouse; Tissue: lymphoid; Tumor: lymphoid neoplasm; Transformed by: methylcholanthrene-induced; Original line: P388 |
| Description | Species: mouse; Tissue: lymphoid; Tumor: lymphoid neoplasm; derived from a tumor induced in vivo by methylcholanthrene; Original line: P388 |
| Depositor | Dr A. Rubartelli, Clinical Pathology Dept, IST, Genoa, Italy |
| Reference paper | Am J Pathol 1957;33:603 |
| Morphology and growth | continuous culture, grown semiadherent, morphology macrophage-like |
| Culture conditions | L-15 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6; seed at 2-4 x 10^4 cells/cm2; 37C |
| Properties | selected for the high production of IL-1; the cells exhibit high effector activity in antibody dependent cell mediated cytotoxicity |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, Pep B |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Cancer Res 1977;37:546-550 - PMID: 318922
J Immunol 1975;114:894-897 - PMID: 1167564 J Immunol 1977;119:950-954 - PMID: 894031 |
| Comments | the cells detach very easily; CO2 is not required |