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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MG-1361 |
| Accession number | ICLC ATL97003 |
| Brief description | Species: mouse, CD-1 transgenic; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: rat activated neu oncogene |
| Description | Species: mouse, CD-1 transgenic male 5 months old; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: rat activated neu oncogene |
| Depositor | Dr M.G. Sacco, Advanced Biomedical Technologies Institute, CNR, Milan, Italy |
| Reference paper | Breast Cancer Res Treat 1998;47:171-180 - DOI: 10.1023/a:1005988715285 - PMID: 9497105 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | William's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split cultures 1:3-1:6 using trypsin/EDTA do not allow to reach confluence; seed at 3-5x104 cells/cm2; 37C, 5% CO2. |
| Properties | estrogen and progestinic receptor; Neu over expression; sensitive to anti-estrogens and apoptosis inducing. |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Passage number | 23 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Gene Ther 1998;5:388-393 - DOI: 10.1038/sj.gt.3300592
- PMID: 9614559
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