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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MiCl1 (S+L-) |
| Accession number | ICLC ATL97001 |
| Brief description | Species: mustela vison (mink); Tissue: lung, fetal; Transformed by: Moloney-MSV; Original line: Mv.1.Lu. |
| Description | Species: mustela vison (mink); Tissue: lung, fetal; Transformed by: Moloney-MSV; Original line: Mv.1.Lu. |
| Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
| Reference paper | Virology 1975;67:288 - DOI: 10.1016/0042-6822(75)90427-4 - PMID: 170737 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA, see at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Viruses | Moloney-MSV |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mink with MD, Pep B |
| Passage number | 13 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Science 1976;192:1143 - DOI: 10.1126/science.179144
- PMID: 179144
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