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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | P3X63Ag8U.1 |
| Accession number | ICLC ATL96012 |
| Brief description | Species: mouse, BALB/c; Tumor: myeloma |
| Description | Species: mouse, BALB/c female; Tumor: myeloma; Derived from: P3X63Ag8 |
| Depositor | Obtained from ECACC. |
| Reference paper | Curr Top Microbiol Immunol 1978;81:1-7 - DOI: 10.1007/978-3-642-67448-8_1 - PMID: 567551 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split 1:5-1:10; maintain cultures between 3 and 9x105 cells/ml; rapid cell death occurs at >1x106 cells/ml. 37C, 5% CO2. |
| Properties | fusion partner; HGPRT- and HAT sensitive; does not synthesize gamma1 chains, produces kappa light chains which are not secreted |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with AST, G6PD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |