Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | S+L-CAT2 |
Accession number | ICLC ATL96011 |
Brief description | Species: cat; Transformed by: murine sarcoma virus |
Description | Species: cat; Transformed by: murine sarcoma virus |
Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | RPMI 1640 or DMEM + 2 mM L-Glutamine + 10% FBS; Split 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37C, 5% CO2. |
Properties | virus replication; virology; the cell line does not produce murine leukemia virus (L-). |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as cat with AST, G6PD |
Passage number | 7 |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Further bibliography | J Gen Virol 1983;64:425-428 - DOI: 10.1099/0022-1317-64-2-425
- PMID: 6300292
J Virol 1974;14:177-179 - DOI: 10.1128/JVI.14.1.177-179.1974 - PMID: 4365331 Virology 1975;67:288-291 - DOI: 10.1016/0042-6822(75)90427-4 - PMID: 170737 |