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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SC-1 |
| Accession number | ICLC ATL96009 |
| Brief description | Species: mouse; Tissue: embryo |
| Description | Species: mouse; Tissue: embryo |
| Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
| Reference paper | Virology 1975;65:128-134 - DOI: 10.1016/0042-6822(75)90013-6 - PMID: 167514 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:4-1:8 using trypsin/EDTA; seed at 2-5 x 104 cells/cm2. 37C, 5% CO2. |
| Properties | virology; Susceptible to: Moloney-MuLV, N-tropic virus, B-tropic virus |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with AST, PepB |
| Passage number | 56 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |