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Cell line nameSC-1
Accession numberICLC ATL96009
Brief descriptionSpecies: mouse; Tissue: embryo
DescriptionSpecies: mouse; Tissue: embryo
DepositorDr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Reference paperVirology 1975;65:128-134 - DOI: 10.1016/0042-6822(75)90013-6 - PMID: 167514
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:4-1:8 using trypsin/EDTA; seed at 2-5 x 104 cells/cm2. 37C, 5% CO2.
Propertiesvirology; Susceptible to: Moloney-MuLV, N-tropic virus, B-tropic virus
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with AST, PepB
Passage number56
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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