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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | DH82 |
| Accession number | ICLC ATL96008 |
| Brief description | Species: dog, golden retriever; Tissue: monocyte-macrophage |
| Description | Species: dog, golden retriever male 10 years old; Tissue: monocyte-macrophage; Derived from: a golden retriever with malignant histiocytosis |
| Depositor | Obtained from ECACC. |
| Reference paper | In Vitro Cell Dev Biol 1988;24:223-229 - DOI: 10.1007/BF02623551 - PMID: 3350786 |
| Morphology and growth | continuous culture, grown as monolayer, morphology macrophage-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-3 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | The cells are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as dog with AST, G6PD, PepB |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |