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Cell line nameDH82
Accession numberICLC ATL96008
Brief descriptionSpecies: dog, golden retriever; Tissue: monocyte-macrophage
DescriptionSpecies: dog, golden retriever male 10 years old; Tissue: monocyte-macrophage; Derived from: a golden retriever with malignant histiocytosis
DepositorObtained from ECACC.
Reference paperIn Vitro Cell Dev Biol 1988;24:223-229 - DOI: 10.1007/BF02623551 - PMID: 3350786
Morphology and growthcontinuous culture, grown as monolayer, morphology macrophage-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-3 x 104 cells/cm2; 37C, 5% CO2
PropertiesThe cells are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as dog with AST, G6PD, PepB
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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