Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | C2-Rev7 |
Accession number | ICLC ATL96007 |
Brief description | Species: rat; Tumor: hepatoma |
Description | Species: rat; Tumor: hepatoma |
Depositor | Obtained from ECACC. |
Reference paper | Cell 1980;19:1043 - DOI: 10.1016/0092-8674(80)90095-1 - PMID: 6247070 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | Coon's modified Ham's F12 + 5% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 9x103 cells/cm2. 37C, 5% CO2. |
Properties | HGPRT negative |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as rat with AST, Pep B |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Further bibliography | Eur J Biochem 1984;145:455-462 - DOI: 10.1111/j.1432-1033.1984.tb08577.x
- PMID: 6334605
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