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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | GH4-C1 |
| Accession number | ICLC ATL96006 |
| Brief description | Species: rat, Wistar-Furth; Tumor: pituitary adenoma |
| Description | Species: rat, Wistar-Furth female 7 months old; Tumor: pituitary adenoma |
| Depositor | Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | Ham's F10 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2 37C, 5% CO2. |
| Properties | The cells were used for studies on growth hormon and prolactin secretion. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with AST, G6PD, PepB |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | J Gen Physiol 1984;83:309-339 - DOI: 10.1085/jgp.83.3.309
- PMID: 6325585
Proc Natl Acad Sci USA 1985;82:4282-4286 - DOI: 10.1073/pnas.82.12.4282 - PMID: 2408278 |
| Comments | After thawing the viability of the cells may be low |