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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | WEHI-164 |
| Accession number | ICLC ATL96004 |
| Brief description | Species: mouse, BALB/c; Tumor: fibrosarcoma; Transformed by: tumor induced by subcutaneous injection of 3-methylcholanthrene |
| Description | Species: mouse, BALB/c; Tumor: fibrosarcoma; Transformed by: tumor induced by subcutaneous injection of 3-methylcholanthrene |
| Depositor | Obtained from ECACC. |
| Reference paper | Proc Soc Exp Biol Med 1973;144:813-818 - DOI: 10.3181/00379727-144-37688 - PMID: 4543620 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA, seed at 2-4 x 104 cells/cm2. 37C, 5% CO2. |
| Properties | tumorigenicity and cytotoxicity studies: highly sensitive to human cytotoxic monocytes, human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) after pretreatment with actinomycin D. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Blood 1985;65:8-14 - PMID: 3155490
J Natl Cancer Inst 1984;72:23-29 - DOI: 10.1093/jnci/72.1.23 - PMID: 6582301 |