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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | GH3 |
| Accession number | ICLC ATL96003 |
| Brief description | Species: rat, Wistar-Furth; Tumor: pituitary tumor |
| Description | Species: rat, Wistar-Furth female 7 months old; Tumor: pituitary tumor |
| Depositor | Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy |
| Reference paper | Endocrinology 1968;82:342 - DOI: 10.1210/endo-82-2-342 - PMID: 4951281 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | Ham's F10 + 10% FBS + 2mM L-Glutamine; the cells are capable of adaptation in suspension culture using MEM. Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
| Properties | Growth hormone, somatotropin and prolactin secretion.; Susceptible to: herpes simplex, vesic. stomatitis (Indiana) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, MPI |
| Tumorigenicity | tumorigenic in syngeneic animals |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | In Vitro 1970;6:180 - DOI: 10.1007/BF02617762
- PMID: 5535574
J Cell Biol 1969;43:432 - DOI: 10.1083/jcb.43.3.432 - PMID: 5389137 |