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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | RT-1 |
| Accession number | ICLC ATL96002 |
| Brief description | Species: rat, Lewis; Tumor: fibrosarcoma; Transformed by: RSV-33 |
| Description | Species: rat, Lewis; Tumor: fibrosarcoma; Transformed by: RSV-33; tumor induced in vivo by RSV-33; Derived from: co-culture of rat embryonic fibroblasts with chicken sarcoma cells |
| Depositor | Obtained from ECACC. |
| Reference paper | Folia Biol (Praha)1992;38:90-102 - PMID: 1326448 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 5% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37C, 5% CO2. |
| Properties | The cells contain the RSV-33 viral genome, but do not express virus specific RNA |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with AST, NP, MD |
| Tumorigenicity | tumorigenic in syngeneic animals |
| Karyology | modal chromosome number 40 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Neoplasma 1991;38:617 - PMID: 1662779
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