Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | GS-9L |
Accession number | ICLC ATL96001 |
Brief description | Species: rat; Tumor: glioma |
Description | Species: rat; Tumor: glioma |
Depositor | Obtained from ECACC |
Reference paper | J Neurosurg 1971;34:335 - DOI: 10.3171/jns.1971.34.3.0335 - PMID: 5547317 |
Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 |
Properties | transplantation; tumor studies |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as rat with AST, MD |
Passage number | 37 |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Further bibliography | Cancer Res 1990;50:138-141 - PMID: 2293547
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