Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | C6 |
Accession number | ICLC ATL95007 |
Brief description | Species: rat; Tumor: glial tumor |
Description | Species: rat; Tumor: glial tumor |
Depositor | Dr T. Florio, Neuroscience Dept, ABC, Genoa Italy. |
Reference paper | Science 1968;161:370 - DOI: 10.1126/science.161.3839.370 - PMID: 4873531 |
Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
Culture conditions | Ham's F12 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 106 cells/cm2. 37C, 5% CO2 |
Properties | S-100 protein production (depending on the level of confluence). HLA: B27.; Susceptible to: vaccinia, herpes simplex, vesic. stomatitis (Indiana) |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as rat with AST, MD, NP |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Further bibliography | Fed Proc 1968;27:720 - DOI: 10.1016/0006-291x(75)90293-4 |