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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

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Cell line nameHTC
Accession numberICLC ATL95006
Brief descriptionSpecies: rat, buffalo; Tissue: ascitic fluid; Tumor: hepatoma
DescriptionSpecies: rat, buffalo male; Tissue: ascitic fluid; Tumor: hepatoma; Derived from: Morris hepatoma 7288C
DepositorObtained from ECACC.
Reference paperProc Natl Acad Sci USA 1966;56:269 - DOI: 10.1073/pnas.56.1.296 - PMID: 4381784
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Propertiesbiochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone phosphate and hydrocortisone receptor
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Tumorigenicitytumorigenic in syngeneic animals
Karyologyextra chromosomes 1 and 13
Freezing mediumCulture medium 70% + 20% Horse serum + 10% DMSO
Further bibliographyAdv Cancer Res 1965;9:227 - DOI: 10.1016/s0065-230x(08)60448-0 - PMID: 4285261
ATLA 1988;16:32 - DOI: 10.1016/0006-291x(75)90293-4

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