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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HTC |
| Accession number | ICLC ATL95006 |
| Brief description | Species: rat, buffalo; Tissue: ascitic fluid; Tumor: hepatoma |
| Description | Species: rat, buffalo male; Tissue: ascitic fluid; Tumor: hepatoma; Derived from: Morris hepatoma 7288C |
| Depositor | Obtained from ECACC. |
| Reference paper | Proc Natl Acad Sci USA 1966;56:269 - DOI: 10.1073/pnas.56.1.296 - PMID: 4381784 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone phosphate and hydrocortisone receptor |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, NP |
| Tumorigenicity | tumorigenic in syngeneic animals |
| Karyology | extra chromosomes 1 and 13 |
| Freezing medium | Culture medium 70% + 20% Horse serum + 10% DMSO |
| Further bibliography | Adv Cancer Res 1965;9:227 - DOI: 10.1016/s0065-230x(08)60448-0
- PMID: 4285261
ATLA 1988;16:32 - DOI: 10.1016/0006-291x(75)90293-4 |