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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameCHO
Accession numberICLC ATL95003
Brief descriptionSpecies: hamster, Chinese; Tissue: ovary
DescriptionSpecies: hamster, Chinese female; Tissue: ovary
DepositorObtained from ECACC
Reference paperJ Exp Med 1958;108:945 - DOI: 10.1016/0006-291x(75)90293-4
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like and epithelial-like
Culture conditionsHam's F12 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA;seed at 1-3x104 cells/cm2;37C, 5% CO2
Propertiesgenetics; toxicity screening; nutrition; gene expression
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as hamster with AST, MD
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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