ICLC logo Cell bank
IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line name3T3
Accession numberICLC ATL95002
Brief descriptionSpecies: mouse, Swiss albino; Tissue: embryo
DescriptionSpecies: mouse, Swiss albino; Tissue: embryo
DepositorObtained from ECACC
Reference paperJ Cell Biol 1963;17:299-313 - DOI: 10.1016/0006-291x(75)90293-4
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
PropertiesContact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with G6PD, MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyVirology 1965;27:179-185 - DOI: 10.1016/0042-6822(65)90157-1 - PMID: 4284655

For any info contact the Interlab Cell Line Collection
HTML by Paolo Romano