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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | L929 |
| Accession number | ICLC ATL95001 |
| Brief description | Species: mouse, C3H/An; Tissue: connective |
| Description | Species: mouse, C3H/An male 100 days old; Tissue: connective |
| Depositor | Obtained from ECACC. |
| Reference paper | J Natl Cancer Inst 1948;9:229 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at1x104 cells/cm2; 37C, 5% CO2. |
| Properties | interferon assay; virus titration; tumor necrosis factor assay; virology; Susceptible to: pseudorabies, herpes simplex, vesic. stomatitis (Indiana), bluetongue virus |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with AST, G6PD, MD, NP, Pep B |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | J Clin Microbiol 1988;26:2324 - DOI: 10.1128/jcm.26.11.2324-2327.1988
- PMID: 2853175
J Natl Cancer Inst 1943;4:165-212 - DOI: 10.1016/0006-291x(75)90293-4 |