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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NULLI-SCC1 |
| Accession number | ICLC ATL06001 |
| Brief description | Species: mouse, 129/SV; Tissue: testis; Tumor: teratocarcinoma |
| Description | Species: mouse, 129/SV male; Tissue: testis; Tumor: teratocarcinoma |
| Depositor | Dr S. Astigiano, Transgenic Animals Dept, IST at ABC, Genoa, Italy |
| Reference paper | Cell 1975 ;5(3):229-43 - DOI: 10.1016/0092-8674(75)90098-7 - PMID: 1097123 |
| Morphology and growth | continuous culture, grown as monolayer, morphology undifferentiated |
| Culture conditions | DMEM (with 4.5 g/l glucose) + 10% Calf serum; split 1:5 - 1:10, 3 times per week; 37C, 5% CO2 |
| Properties | pluripotent; differentiation |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | 95% FCS + 5% DMSO |
| Validation assays | Multiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers |
| Comments | Growth on gelatin-coated dishes. Differentiation into visceral or parietal endoderm with retinoic acid or HMBA treatment |