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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | L6H2 |
| Accession number | ICLC ATL00001 |
| Brief description | Species: rat; Transformed by: non myogenic subclone; Original line: L6 (ATCC CRL1458) |
| Description | Species: rat newborn; spontaneously transformed; non myogenic subclone of the rat myogenic L6 cell line; Original line: L6 (ATCC CRL1458) |
| Depositor | Dr S. Adamo, Medical Istology Embriology Dept, University La Sapienza, Rome, Italy |
| Reference paper | Am J Physiol 1993;265:C113-C121 - DOI: 10.1152/ajpcell.1993.265.1.C113 - PMID: 8393277 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like and round cells lightly adherent |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split cultures 1:10-1:20 using Trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | L6H2 display limited adhesion, do not give rise to myotubes, grow in soft agar |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, NP |
| Passage number | 7 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |