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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | E.Derm |
| Accession number | ICLC AL97004 |
| Brief description | Species: horse; Tissue: dermis |
| Description | Species: horse female 4 years old; Tissue: dermis |
| Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2. Finite lifespan of approximately 40 passages. |
| Properties | Susceptible to: herpes simplex, reovirus 3, coronavirus, equine infectious anaemia virus, vesicular stomatitis (Ogden strain) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as horse with NP |
| Passage number | 20 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Am J Vet Res 1981;42:1501-1505 - PMID: 6275755
Am J Vet Res 1986;47:1931-1934 - PMID: 3021027 Int J Cancer 1975;15:171-179 - DOI: 10.1002/ijc.2910150202 - PMID: 165152 |