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Cell line nameCf2Th
Accession numberICLC AL97003
Brief descriptionSpecies: dog; Tissue: thymus
DescriptionSpecies: dog; Tissue: thymus
DepositorDr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Reference paperIn Vitro 1976;12:665 - DOI: 10.1007/BF02797468 - PMID: 190163
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2
PropertiesPermissive for xenotropic viruses found in Balb/c and AKR mice; C-type viruses growth; Susceptible to: reovirus, pseudorabies, herpes simplex, parvovirus, calicivirus, feline rhinotracheitis, retroviruses
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as dog with G6PD, MD.
Passage number9
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyAm J Vet Res 1983;44:865 - PMID: 6869994
In Vitro 1977;9:176 - DOI: 10.1016/0006-291x(75)90293-4

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