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Cell line nameLTK-
Accession numberICLC AL97001
Brief descriptionSpecies: mouse, C3H/An; Tissue: connective
DescriptionSpecies: mouse, C3H/An male 100 days old; Tissue: connective; Derived from: L-M (ATCC CCL1.2)
DepositorDr T. Florio, Neuroscience Unit, ABC, Genoa, Italy.
Reference paperExp Cell Res 1963;31:297-312 - DOI: 10.1016/0006-291x(75)90293-4
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin /EDTA; seed at 5x103-2x104 cells/cm2; 37C, 5% CO2.
Propertiesresistant to 5-BUdR;thymidine kinase mutant; biochemical and genetic studies, including somatic cell hybridization and gene transfer
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with AST, G6PD
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCell 1977;11:223 - DOI: 10.1016/0092-8674(77)90333-6 - PMID: 194704
Proc Natl Acad Sci USA 1972;69:510-514 - DOI: 10.1073/pnas.69.2.510 - PMID: 4110482
Proc Natl Acad Sci USA 1980;77:1583-1587 - DOI: 10.1073/pnas.77.3.1583 - PMID: 6929511

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