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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | LTK- |
| Accession number | ICLC AL97001 |
| Brief description | Species: mouse, C3H/An; Tissue: connective |
| Description | Species: mouse, C3H/An male 100 days old; Tissue: connective; Derived from: L-M (ATCC CCL1.2) |
| Depositor | Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy. |
| Reference paper | Exp Cell Res 1963;31:297-312 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin /EDTA; seed at 5x103-2x104 cells/cm2; 37C, 5% CO2. |
| Properties | resistant to 5-BUdR;thymidine kinase mutant; biochemical and genetic studies, including somatic cell hybridization and gene transfer |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with AST, G6PD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Cell 1977;11:223 - DOI: 10.1016/0092-8674(77)90333-6
- PMID: 194704
Proc Natl Acad Sci USA 1972;69:510-514 - DOI: 10.1073/pnas.69.2.510 - PMID: 4110482 Proc Natl Acad Sci USA 1980;77:1583-1587 - DOI: 10.1073/pnas.77.3.1583 - PMID: 6929511 |