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Interlab Cell Line Collection (ICLC)
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Cell line nameBRL-3A
Accession numberICLC AL00002
Brief descriptionSpecies: rat, buffalo; Tissue: liver
DescriptionSpecies: rat, buffalo female 5 weeks old; Tissue: liver
DepositorDr T. Russo, Biochemistry and Medical Biotechnology Dept, Naples, Italy
Reference paperCarnegie Inst. Washington Yearbook 1968;67:419-421
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsCoon's F12 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5%CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with NP, PepB
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCell 1977;11:441-446 - DOI: 10.1016/0092-8674(77)90062-9 - PMID: 302146
Proc Natl Acad Sci USA 1969;62:852-859 - DOI: 10.1073/pnas.62.3.852 - PMID: 4308097

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