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Cell line nameL6C5
Accession numberICLC AL00001
Brief descriptionSpecies: rat; Tissue: skeletal muscle
DescriptionSpecies: rat newborn; Tissue: skeletal muscle; Derived from: L6 cell line (ATCC CRL 1458)
DepositorDr S. Adamo, Medical Istology Embriology Dept, University La Sapienza, Rome, Italy
Reference paperAm J Physiol 1993;265:C113-C121 - DOI: 10.1152/ajpcell.1993.265.1.C113 - PMID: 8393277
Morphology and growthcontinuous culture, grown as monolayer, morphology myoblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesMyogenic, vasopressin responsive. Differentiation is induced by reduction of serum, IGF-1, vasopressin (AVP). AVP stimulates PLC, PLD, cAMP-phosphodiesterase. Skeletal muscle myosin, creatine kinase, myogenin, vasopressin receptor V1a, IGF-1 receptor, nicotinic acetylcholine receptor subunits.
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Passage number10
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCell Growth Diff 1995;6:81-89 - PMID: 7718487
Cell Growth Diff 1998;9:155-164 - PMID: 9486852
J Cell Physiol 1997;171:34-42 - DOI: 10.1002/(SICI)1097-4652(199704)171:1<34::AID-JCP5>3.0.CO;2-I - PMID: 9119890
Lipids 1999;34:581-582 Mol Biol Cell 1999;10(12)in press
CommentsL6C5 fuse in culture to form multinucleated myotubes. The myogenic potential is lost if cells are allowed to become confluent.

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